Wednesday, January 6, 2010

Why would different restriction enzymes cut the same dna molecule into different numbers of fragments?

The enzymes recognise different restriction sites. The same DNA molecule may have only one restriction site recognised by Enzyme A, but two by Enzyme B, and hence a different number of fragments would be produced.Why would different restriction enzymes cut the same dna molecule into different numbers of fragments?
Each restriction enzyme recognizes a different sequence of bases to cut at, and any piece of DNA will have some variable number of possible recognition sites for each enzyme, ranging from 0 to some larger number (some sites are common, some are very rare).Why would different restriction enzymes cut the same dna molecule into different numbers of fragments?
it depends on the DNA sample wat u take into consideration......


1. May be the DNA have more than one cleavage site of same restriction enzyme so that it cut into various frgments


2. or there r more than one restriction site r present so various fragments r produced of different length.......

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